Alix Antibody (7E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26435

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26435-100ul
NBP3-26435-50ul
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7E9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Alix Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Alix [UniProt Q8WUM4]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alix Antibody (7E9)

Alix Antibody (7E9)

Western Blot: Alix Antibody (7E9) [NBP3-26435] -

Western Blot: Alix Antibody (7E9) [NBP3-26435] - Positive Western Blot detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate.
All lanes: Alix Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 97, 97, 31 kDa
Observed band size: 97 kDa
Alix Antibody (7E9)

Flow Cytometry: Alix Antibody (7E9) [NBP3-26435] -

Flow Cytometry: Alix Antibody (7E9) [NBP3-26435] - Overlay histogram showing Jurkat cells stained with Alix Antibody (7E9) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Alix Antibody (7E9)

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Alix

Alix encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.

Alternate Names

AIP1DRIP4, ALG-2 interacting protein 1, ALG-2 interacting protein X, ALG-2-interacting protein 1, alinx, ALIX, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4, Hp95, KIAA1375, MGC17003, PDCD6-interacting protein, programmed cell death 6 interacting protein, programmed cell death 6-interacting protein

Gene Symbol

PDCD6IP

Additional Alix Products

Product Documents for Alix Antibody (7E9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Alix Antibody (7E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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