Alix Antibody (7E9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26435
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
ELISA, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7E9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Alix Antibody (7E9)
Immunogen
A synthesized peptide derived from Human Alix [UniProt Q8WUM4]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Alix Antibody (7E9)
Western Blot: Alix Antibody (7E9) [NBP3-26435] -
Western Blot: Alix Antibody (7E9) [NBP3-26435] - Positive Western Blot detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate.All lanes: Alix Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 97, 97, 31 kDa
Observed band size: 97 kDa
Flow Cytometry: Alix Antibody (7E9) [NBP3-26435] -
Flow Cytometry: Alix Antibody (7E9) [NBP3-26435] - Overlay histogram showing Jurkat cells stained with Alix Antibody (7E9) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for Alix Antibody (7E9)
Application
Recommended Usage
Flow Cytometry
1:20-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Alix
Alternate Names
AIP1DRIP4, ALG-2 interacting protein 1, ALG-2 interacting protein X, ALG-2-interacting protein 1, alinx, ALIX, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4, Hp95, KIAA1375, MGC17003, PDCD6-interacting protein, programmed cell death 6 interacting protein, programmed cell death 6-interacting protein
Gene Symbol
PDCD6IP
Additional Alix Products
Product Documents for Alix Antibody (7E9)
Product Specific Notices for Alix Antibody (7E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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