ALK-1 Antibody (8B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26524

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ALK-1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human ALK-1 [UniProt P37023]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ALK-1 Antibody (8B6)

ALK-1 Antibody (8B6)

Immunohistochemistry: ALK-1 Antibody (8B6) [NBP3-26524] -

Immunohistochemistry: ALK-1 Antibody (8B6) [NBP3-26524] - Image of ALK-1 Antibody (8B6) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
ALK-1 Antibody (8B6)

Western Blot: ALK-1 Antibody (8B6) [NBP3-26524] -

Western Blot: ALK-1 Antibody (8B6) [NBP3-26524] - Positive Western Blot detected in: Daudi whole cell lysate, U87 whole cell lysate.
All lanes: ALK-1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 57 kDa
Observed band size: 65 kDa

Applications for ALK-1 Antibody (8B6)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ALK-1

LTK, ALK and Ros have been identified as receptor tyrosine kinases having sequence similarity to the insulin receptor subfamily of kinases. LTK (leukocyte tyrosine kinase) is expressed in murine B lymphocyte precursors and has also been found in forebrain neurons. ALK (anaplastic lymphoma kinase) is normally highly expressed, specifically in the nervous system. A truncated form containing the catalytic domian of ALK is expressed as the result of a translocation occurring in many non-Hodgkin's lymphomas. The c-Ros gene was originally identified in mutant form as an oncogene. Ros is normally expressed in a small number of epithelial cell types and may play a role in epithelial development.

Long Name

Activin Receptor-like Kinase 1

Alternate Names

ACVRL1, ALK1

Gene Symbol

ACVRL1

Additional ALK-1 Products

Product Documents for ALK-1 Antibody (8B6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ALK-1 Antibody (8B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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