Alkaline Phosphatase/ALPL Antibody (10B1M5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15273
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10B1M5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Alkaline Phosphatase/ALPL (P05186). MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKT
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Alkaline Phosphatase/ALPL Antibody (10B1M5)
Western Blot: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273]
Western Blot: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273] - Western blot analysis of extracts of various cell lines, using Alkaline Phosphatase/ALPL (ALPL) Rabbit mAb (NBP3-15273) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273]
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273] - Immunohistochemistry of paraffin-embedded human placenta using Alkaline Phosphatase/ALPL (ALPL) Rabbit mAb (NBP3-15273) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Alkaline Phosphatase/ALPL Antibody (10B1M5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase/ALPL
Long Name
Alkaline Phosphatase Liver
Alternate Names
Akp2, AP-TNAP, HOPS, TNAP, TNSALP
Gene Symbol
ALPL
Additional Alkaline Phosphatase/ALPL Products
Product Documents for Alkaline Phosphatase/ALPL Antibody (10B1M5)
Product Specific Notices for Alkaline Phosphatase/ALPL Antibody (10B1M5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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