Alkaline Phosphatase/ALPP Antibody (H17E2)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-750
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # H17E2
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Placental membrane
Localization
Attached to the membrane by a GPI-anchor.
Specificity
This recognises human placental alkaline phosphate. The has been widely used in immunolocalisation studies of germ cell tumours and may also be used in ELISA based assays for the measurement of soluble PLAP.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Alkaline Phosphatase/ALPP Antibody (H17E2)
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
10 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. This antibody does not work in Immunohistochemistry-Paraffin.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
UniProt
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Alkaline Phosphatase/ALPP Antibody (H17E2)
Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (H17E2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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