Alkaline Phosphatase/ALPP Antibody (JM22-53)

Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]

Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of Placental alkaline phosphatase/ALPP on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase/ALPP antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]

Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Staining PLAP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human seminoma tissue with Rabbit anti-Placental alkaline phosphatase/ALPP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.