Alkaline Phosphatase/ALPP Antibody (SPM593)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44955
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (SPM593) [NBP2-44955]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-SPM593-Immunohistochemistry-Paraffin-NBP2-44955-img0001.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (SPM593) [NBP2-44955]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SPM593
Concentration
0.2 mg/ml
Product Summary for Alkaline Phosphatase/ALPP Antibody (SPM593)
Immunogen
Recombinant full-length human Alkaline Phosphatase/ALPP protein (Uniprot: P05187)
Localization
Cytoplasmic and cell surface
Specificity
Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Marker
Germ Cell Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47989)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Alkaline Phosphatase/ALPP Antibody (SPM593)
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (SPM593) [NBP2-44955]
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (SPM593) [NBP2-44955] - Human Placenta stained with PLAP Monoclonal Antibody (SPM593).Applications for Alkaline Phosphatase/ALPP Antibody (SPM593)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
UniProt
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Alkaline Phosphatase/ALPP Antibody (SPM593)
Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (SPM593)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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