Alkaline Phosphatase/ALPP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34056
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Alkaline Phosphatase/ALPP Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NWYSDADVPASARQEGCQDIATQLISNMDI
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Alkaline Phosphatase/ALPP Antibody
![Western Blot: Alkaline Phosphatase/ALPP Antibody [NBP2-34056]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-Western-Blot-NBP2-34056-img0005.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody [NBP2-34056]")
Western Blot: Alkaline Phosphatase/ALPP Antibody [NBP2-34056]
Western Blot: Alkaline Phosphatase/ALPP Antibody [NBP2-34056] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15,10. Lane 2: Placenta![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody [NBP2-34056]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34056-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody [NBP2-34056]")
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody [NBP2-34056]
Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody [NBP2-34056] - Staining of human cell line HeLa shows localization to plasma membrane. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody [NBP2-34056]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-Immunohistochemistry-Paraffin-NBP2-34056-img0004.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody [NBP2-34056]")
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody [NBP2-34056]
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody [NBP2-34056] - Staining in human placenta shows strong cytoplasmic and membranous staining in trophoblasts.Applications for Alkaline Phosphatase/ALPP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
UniProt
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Alkaline Phosphatase/ALPP Antibody
Product Specific Notices for Alkaline Phosphatase/ALPP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...