Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25355
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Drosophila
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1268
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary
Immunogen
Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase, Intestinal. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%), Bovine (85%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images
Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] -
Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] -
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] -
Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase, Intestinal
Long Name
Alkaline Phosphatase, Intestinal Type
Alternate Names
alkaline phosphatase, intestinal, alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme
Gene Symbol
ALPI
Additional Alkaline Phosphatase, Intestinal Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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