Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22193

Novus Biologicals, part of Bio-Techne
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NBP2-22193-0.025ml
NBP2-22193
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rabbit

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F4

Format

BSA Free

Concentration

1 mg/ml

View all Alkaline Phosphatase, Tissue Non-Specific Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Alkaline Phosphatase, Tissue Non-Specific expressed in E. Coli.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4)BSA Free [NBP2-22193]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4)BSA Free [NBP2-22193]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Primary antibody dilution: 1:1000. Image from verified customer review.
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of ovarian cancer tissues using Alkaline Phosphatase, Tissue Non-Specific mouse mAb with DAB staining.
Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Image from verified customer reviews.
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Applications for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-22193 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Tissue Non-Specific

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Gene Symbol

ALPL

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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