Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free

Validated:

Cited:

Validated:

Cited:

Unconjugated

Monoclonal Mouse IgG₁ Clone # TRA-2-49

BSA Free

1.0 mg/ml

2102Ep Human Embryonal Carcinoma Cells

Liver/Bone/Kidney Alkaline Phosphatase

Reactivity has been demonstrated with Liver/ Bone/Kidney isozyme of Alkaline Phosphatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon), New World Monkeys (Owl Monkey, Squirrel Monkey), Cat, Pig, Rabbit and Tiger.

No reactivity has been observed with Old World Monkeys (Baboon, Rhesus monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey), Bovine, Canine, Goat, Guinea Pig, Hamster, Mouse, Rat and Sheep. This antibody does not cross react with other isozymes of AP.

Cell Membrane

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells. This antibody does not cross react with other isozymes of AP.

Monoclonal

Mouse

IgG₁

Protein A purified

20mM PBS (pH 7.6) and 0.25M NaCl

BSA Free

0.1% Sodium Azide

1.0 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.