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Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47996

Novus Biologicals, part of Bio-Techne
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NBP2-47996-0.1mg

Key Product Details

Species Reactivity

Human, Bovine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # V17.1

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Bovine alkaline phosphatase (Uniprot: P05186)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44962).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) - Azide and BSA Free [NBP2-47996]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) - Azide and BSA Free [NBP2-47996]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) - Azide and BSA Free [NBP2-47996] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1).

Applications

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Frozen

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Tissue Non-Specific

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Gene Symbol

ALPL

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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