Allophycocyanin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8927

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Multi-Species

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 936809

View all Allophycocyanin Antibodies »

Product Specifications

Immunogen

Allophycocyanin isolated from red algae
Accession # P25054

Specificity

Detects Allophycocyanin in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Allophycocyanin Antibody

Detection of Allophycocyanin (APC) antibody in Human PBMCs stained with APC-Conjugated Anti-Human CD3e antibody by Flow Cytometry.

Detection of Allophycocyanin (APC) in Human PBMCs stained with APC-Conjugated Anti-Human CD3e by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMCs) stained with APC-conjugated Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # FAB100A) were stained with Mouse Anti-Allophycocyanin Monoclonal Antibody (Catalog # MAB8927, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Allophycocyanin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cell (PBMCs) stained with APC-conjugated Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # FAB100A)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Allophycocyanin

The APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cy5.5 and APC/Cy7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with tested mouse and human cells. The APC003 antibody can be used for separation of APC-antibody labeled cells or as a secondary step to amplify APC conjugated primary antibody signals (i.e. biotinylated APC003 as secondary step, followed by Streptavidin-APC).

Alternate Names

APC

UniProt

P25054

Additional Allophycocyanin Products

Product Documents for Allophycocyanin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Allophycocyanin Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.