alpha 1-Acid Glycoprotein Antibody (2F9-1F10)
Novus Biologicals, part of Bio-Techne | Catalog # H00005004-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2F9-1F10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ORM1 (AAH26238, 18 a.a. ~ 201 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AQIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES
Specificity
ORM1 - orosomucoid 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for alpha 1-Acid Glycoprotein Antibody (2F9-1F10)
Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]
Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody (M01), clone 2F9-1F10.Lane 1: ORM1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.Immunohistochemistry-Paraffin: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]
Immunohistochemistry-Paraffin: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. Antibody concentration 1 ug/ml.Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]
Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi ( Cat # H00005004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody (M01), clone 2F9-1F10 (Cat # H00005004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for alpha 1-Acid Glycoprotein Antibody (2F9-1F10)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:1000
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: alpha 1-Acid Glycoprotein
Alternate Names
AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid
Entrez Gene IDs
5004 (Human)
Gene Symbol
ORM1
OMIM
138600 (Human)
UniProt
Additional alpha 1-Acid Glycoprotein Products
Product Documents for alpha 1-Acid Glycoprotein Antibody (2F9-1F10)
Product Specific Notices for alpha 1-Acid Glycoprotein Antibody (2F9-1F10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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