alpha 2-Macroglobulin Antibody (2B5)
Novus Biologicals, part of Bio-Techne | Catalog # H00000002-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2B5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A2M (NP_000005.2, 641 a.a. ~ 730 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTET
Specificity
A2M - alpha-2-macroglobulin (2B5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for alpha 2-Macroglobulin Antibody (2B5)
![Proximity Ligation Assay: alpha 2-Macroglobulin Antibody (2B5) [H00000002-M03]](https://resources.bio-techne.com/images/products/alpha-2-Macroglobulin-Antibody-2B5-Proximity-Ligation-Assay-H00000002-M03-img0001.jpg "Proximity Ligation Assay: alpha 2-Macroglobulin Antibody (2B5) [H00000002-M03]")
Proximity Ligation Assay: alpha 2-Macroglobulin Antibody (2B5) [H00000002-M03]
Proximity Ligation Assay: alpha 2-Macroglobulin Antibody (2B5) [H00000002-M03] - Analysis of protein-protein interactions between IL1B and A2M. HeLa cells were stained with anti-IL1B rabbit purified polyclonal 1:1200 and anti-A2M mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).![ELISA: alpha 2-Macroglobulin Antibody (2B5) [H00000002-M03]](https://resources.bio-techne.com/images/products/alpha-2-Macroglobulin-Antibody-2B5-ELISA-H00000002-M03-img0003.jpg "ELISA: alpha 2-Macroglobulin Antibody (2B5) [H00000002-M03]")
ELISA: alpha 2-Macroglobulin Antibody (2B5) [H00000002-M03]
ELISA: alpha 2-Macroglobulin Antibody (2B5) [H00000002-M03] - Detection limit for recombinant GST tagged A2M is 3 ng/ml as a capture antibody.Applications for alpha 2-Macroglobulin Antibody (2B5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: alpha 2-Macroglobulin
Additional alpha 2-Macroglobulin Products
Product Documents for alpha 2-Macroglobulin Antibody (2B5)
Product Specific Notices for alpha 2-Macroglobulin Antibody (2B5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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