alpha-2A Adrenergic R/ADRA2A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2819

Novus Biologicals, part of Bio-Techne
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NB100-2819
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all alpha-2A Adrenergic R/ADRA2A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TERRPNGLGPERS corresponding to internal region according to NP_000672.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for alpha-2A Adrenergic R/ADRA2A Antibody

Immunocytochemistry/ Immunofluorescence: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819]

Immunocytochemistry/ Immunofluorescence: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819]

Immunocytochemistry/Immunofluorescence: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819]

Flow Cytometry: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819]

Flow Cytometry: alpha-2A Adrenergic R/ADRA2A Antibody [NB100-2819] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for alpha-2A Adrenergic R/ADRA2A Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-2A Adrenergic R/ADRA2A

This study investigates the involvement of alpha2-adrenergic receptors (AR) in mouse brain induced by a low dose of methamphetamine (METH, 2 mg/kg). Immunohistochemical studies show that alpha2A-AR increased in the dentate gyrus area of the hippocampus 24 h after five repeated administrations of METH. The hippocampal alpha2A-AR proteins rose 3.2-fold when compared to the saline-administered mice. The other adrenergic receptor, alpha1D-AR, were not changed by the treatment. Moreover, alphao-subunits of GTP-binding proteins (Galphao), one of the downstream molecules of alpha2A-AR, was also increased by the treatment. These suggest that the repeated administration of low-doses of METH causes quantitative changes of the signaling of alpha2A-AR in the mouse hippocampus.

Long Name

Alpha-2A Adrenergic Receptor

Alternate Names

a-2A AdrenergicR, ADRA2A, alpha2A AdrenergicR, ZNF32

Entrez Gene IDs

150 (Human); 11551 (Mouse)

Gene Symbol

ADRA2A

UniProt

NP_000672.2 (Human)

Additional alpha-2A Adrenergic R/ADRA2A Products

Product Documents for alpha-2A Adrenergic R/ADRA2A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-2A Adrenergic R/ADRA2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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