alpha-Actinin 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85791
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat, Canine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKV
Reactivity Notes
Reactivity reported in scientific literature (PMID: 23435261). Canine reactivity per customer review.
Marker
Z-disk Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for alpha-Actinin 1 Antibody
Western Blot: alpha-Actinin 1 Antibody [NBP1-85791]
Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791]
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791] - Staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.Western Blot: alpha-Actinin 1 Antibody [NBP1-85791]
Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissueApplications for alpha-Actinin 1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 2 reviews rated 3 using NBP1-85791 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha Actinin 1
Long Name
Actinin alpha 1
Alternate Names
ACTN1, alpha-Actinin 1, alphaActinin 1, BDPLT15
Gene Symbol
ACTN1
Additional alpha Actinin 1 Products
Product Documents for alpha-Actinin 1 Antibody
Product Specific Notices for alpha-Actinin 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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