Alpha Actinin 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15356

Novus Biologicals, part of Bio-Techne
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NBP2-15356
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Xenopus (94%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Alpha Actinin 3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alpha Actinin 3 Antibody

Western Blot: Alpha Actinin 3 Antibody [NBP2-15356]

Western Blot: Alpha Actinin 3 Antibody [NBP2-15356]

Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.
Western Blot: Alpha Actinin 3 Antibody [NBP2-15356]

Western Blot: Alpha Actinin 3 Antibody [NBP2-15356]

Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:10000.
Western Blot: Alpha Actinin 3 Antibody [NBP2-15356]

Western Blot: Alpha Actinin 3 Antibody [NBP2-15356]

Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE; antibody diluted at 1:10000.
View 2 more images

Applications for Alpha Actinin 3 Antibody

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Alpha Actinin 3

Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments [provided by RefSeq]

Alternate Names

actinin, alpha 3, alpha-actinin skeletal muscle, Alpha-actinin skeletal muscle isoform 3, alpha-actinin-3, F-actin cross-linking protein, MGC117002, MGC117005

Gene Symbol

ACTN3

UniProt

Q08043 (Human)

Additional Alpha Actinin 3 Products

Product Documents for Alpha Actinin 3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Alpha Actinin 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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