Alpha Actinin 4 Antibody (S06-3D7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14888

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14888-100ul
NBP3-14888-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S06-3D7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Alpha Actinin 4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Alpha Actinin 4 (Uniprot#: O43707)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alpha Actinin 4 Antibody (S06-3D7)

Western Blot: Alpha Actinin 4 Antibody (S06-3D7) [NBP3-14888]

Western Blot: Alpha Actinin 4 Antibody (S06-3D7) [NBP3-14888]

Western Blot: Alpha Actinin 4 Antibody (S06-3D7) [NBP3-14888] - Western blot detection of Alpha Actinin 4 in K562, Rat Brain, C6, 3T3, Hela cell lysates using Alpha Actinin 4 Rabbit mAb (1:1000 diluted). Predicted band size: 105kDa. Observed band size: 105kDa.
Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody (S06-3D7) [NBP3-14888]

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody (S06-3D7) [NBP3-14888]

Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody (S06-3D7) [NBP3-14888] - Immunofluorescence of Alpha Actinin 4 (green) in Hela cells using Alpha Actinin 4 Rabbit mAb at dilution 1/200, and DAPI (blue)

Applications for Alpha Actinin 4 Antibody (S06-3D7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/50

Western Blot

1/1000-1/5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alpha Actinin 4

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Alternate Names

actinin alpha4 isoform, actinin, alpha 4, ACTININ-4, alpha-actinin-4, DKFZp686K23158, F-actin cross-linking protein, focal segmental glomerulosclerosis 1, FSGS, FSGS1, Non-muscle alpha-actinin 4

Gene Symbol

ACTN4

Additional Alpha Actinin 4 Products

Product Documents for Alpha Actinin 4 Antibody (S06-3D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Alpha Actinin 4 Antibody (S06-3D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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