Alpha Actinin 4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15358

Novus Biologicals, part of Bio-Techne
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NBP2-15358
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Alpha Actinin 4 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha Actinin 4. The exact sequence is proprietary.

Localization

Nucleus; Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alpha Actinin 4 Antibody

Western Blot: Alpha Actinin 4 Antibody [NBP2-15358]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15358]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15358] - Alpha Actinin 4 antibody detects alpha Actinin 4 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 4 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody [NBP2-15358]

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody [NBP2-15358]

Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody [NBP2-15358] - Sample: MCF-7 cells were fixed in iced-cold MeOH for 5 min. Green: ACTN4 protein stained by alpha Actinin 4 antibody, N-term diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: Alpha Actinin 4 Antibody [NBP2-15358]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15358]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15358] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:1000.
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Applications for Alpha Actinin 4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Alpha Actinin 4

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]

Alternate Names

actinin alpha4 isoform, actinin, alpha 4, ACTININ-4, alpha-actinin-4, DKFZp686K23158, F-actin cross-linking protein, focal segmental glomerulosclerosis 1, FSGS, FSGS1, Non-muscle alpha-actinin 4

Gene Symbol

ACTN4

UniProt

O43707 (Human)

Additional Alpha Actinin 4 Products

Product Documents for Alpha Actinin 4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Alpha Actinin 4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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