alpha-Defensin 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27562
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DESLAPKHPGSRKN corresponding to internal region according to NP_004075.1, NP_005208.1.
Specificity
This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for alpha-Defensin 1 Antibody
Western Blot: alpha-Defensin 1 Antibody [NBP2-27562]
Western Blot: alpha-Defensin 1 Antibody [NBP2-27562] - Analysis of Human Spleen lysate (35ug protein in RIPA buffer) using NBP2-27562 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: alpha-Defensin 1 Antibody [NBP2-27562]
Immunohistochemistry-Paraffin: alpha-Defensin 1 Antibody [NBP2-27562] - (3.8 ug/ml) Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for alpha-Defensin 1 Antibody
Application
Recommended Usage
Immunohistochemistry
4 - 6 ug/ml
Immunohistochemistry-Paraffin
4 - 6 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa band according to NP_004075.1). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Thymus shows staining of selected cells.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: alpha-Defensin 1
Alternate Names
alphaDefensin 1, DEF1, DEFA1, HNP-1, HP1, MRS
Gene Symbol
DEFA1
UniProt
Additional alpha-Defensin 1 Products
Product Documents for alpha-Defensin 1 Antibody
Product Specific Notices for alpha-Defensin 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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