Alpha Dystroglycan Antibody (2238)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49634
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Rabbit
Cited:
Human, Mouse
Applications
Validated:
Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 2238
Concentration
0.1 mg/ml
Product Specifications
Immunogen
Monoclonal antibody 2238 recognizes a glycoepitope unique to brain alpha-dystroglycan.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
The monoclonal antibody 2238 is specific for a glycoepitope on brain bovine alpha-dystroglycan, which is absent on alpha-dystroglycan expressed in all other tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Alpha Dystroglycan Antibody (2238)
Immunohistochemistry: Alpha Dystroglycan Antibody (2238) [NBP1-49634]
Immunohistochemistry: Alpha Dystroglycan Antibody (2238) [NBP1-49634] - Endothelial B-catenin GOF does not affect astrocytic endfoot polarization of a-dystroglycan (a-Dag) and Kir4.1 within the subfornical organ (SFO). Striatal BBB-vessel showing a polarized distribution of a-Dag and Kir4.1 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Dashed lines outline SFO vessels. Scale bar show 2 um (A), 50 um (B), 10 um (C). Image collected and cropped by CiteAb from the following publication (elifesciences.org/articles/43818), licensed under a CC-BY license.Immunohistochemistry: Alpha Dystroglycan Antibody (2238) [NBP1-49634] -
Endothelial beta-catenin GOF does not affect astrocytic endfoot polarization of alpha-dystroglycan ( alpha-Dag) and Kir4.1 within the subfornical organ (SFO).Striatal BBB-vessel showing a polarized distribution of alpha-Dag and Kir4.1 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Dashed lines outline SFO vessels. Scale bar show 2 µm (A), 50 µm (B), 10 µm (C).Immunocytochemistry/ Immunofluorescence: Alpha Dystroglycan Antibody (2238) [NBP1-49634] -
Immunocytochemistry/ Immunofluorescence: Alpha Dystroglycan Antibody (2238) [NBP1-49634] - Endothelial beta-catenin GOF does not affect astrocytic endfoot polarization of alpha-dystroglycan ( alpha-Dag) & Kir4.1 within the subfornical organ (SFO).Striatal BBB-vessel showing a polarized distribution of alpha-Dag & Kir4.1 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Dashed lines outline SFO vessels. Scale bar show 2 µm (A), 50 µm (B), 10 µm (C). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30932814), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Alpha Dystroglycan Antibody (2238)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
This antibody works in Western Blot, Immunoassay and Immunohistochemistry-Paraffin WB:it recognizes alpha-DG as protein of ~130 kD, especially after enrichment of the lysates for dystroglycans Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27015747)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Alpha Dystroglycan
Alternate Names
A3a, AGRNR, DAG156DAG, dystroglycan, dystroglycan 1 (dystrophin-associated glycoprotein 1), Dystrophin-associated glycoprotein 1
Gene Symbol
DAG1
UniProt
Additional Alpha Dystroglycan Products
Product Documents for Alpha Dystroglycan Antibody (2238)
Product Specific Notices for Alpha Dystroglycan Antibody (2238)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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