Alpha Dystroglycan Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52049

Novus Biologicals, part of Bio-Techne
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NBP1-52049
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Alpha Dystroglycan Antibodies »

Product Summary for Alpha Dystroglycan Antibody

Immunogen

Peptide with sequence C-HVGKHEYFMHATDK corresponding to internal region according to NP_004384.4.

Epitope

C-HVGKHEYFMHATDK

Specificity

This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Alpha Dystroglycan Antibody

Western Blot: Alpha Dystroglycan Antibody [NBP1-52049]

Western Blot: Alpha Dystroglycan Antibody [NBP1-52049]

Western Blot: Alpha Dystroglycan Antibody [NBP1-52049] - Staining of Mouse and Rat Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Alpha Dystroglycan Antibody [NBP1-52049]

Immunohistochemistry-Paraffin: Alpha Dystroglycan Antibody [NBP1-52049]

Immunohistochemistry-Paraffin: Alpha Dystroglycan Antibody [NBP1-52049] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Alpha Dystroglycan Antibody [NBP1-52049]

Western Blot: Alpha Dystroglycan Antibody [NBP1-52049]

Western Blot: Alpha Dystroglycan Antibody [NBP1-52049] - Staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 0.2 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Alpha Dystroglycan Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 100 kDa band observed in human skeletal muscle lysates (calculated MW of 97.4 kDa band according to NP_004384.4). Approx. 80 kDa band observed in mouse and rat skeletal muscle lysates (calculated MW of 96.9 kDa band according to NP_034147.1). The observed molecular weights correspond to the glycosylated form of alpha-dystroglycan.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Alpha Dystroglycan

The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3)

Alternate Names

A3a, AGRNR, DAG156DAG, dystroglycan, dystroglycan 1 (dystrophin-associated glycoprotein 1), Dystrophin-associated glycoprotein 1

Entrez Gene IDs

1605 (Human)

Gene Symbol

DAG1

UniProt

NP_004384.3 (Human)

Additional Alpha Dystroglycan Products

Product Documents for Alpha Dystroglycan Antibody

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Product Specific Notices for Alpha Dystroglycan Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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