alpha-Fetoprotein/AFP Antibody (003)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90069

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 003

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all alpha-Fetoprotein/AFP Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human alpha-Fetoprotein/AFP (Uniprot#: P02771; Met1-Val609).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for alpha-Fetoprotein/AFP Antibody (003)

Western Blot: alpha-Fetoprotein/AFP Antibody (003) [NBP2-90069]

Western Blot: alpha-Fetoprotein/AFP Antibody (003) [NBP2-90069]

Western Blot: alpha-Fetoprotein/AFP Antibody (003) [NBP2-90069] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:69 kDaObserved band size:69 kDa
Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (003) [NBP2-90069]

Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (003) [NBP2-90069]

Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (003) [NBP2-90069] - Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-AFP rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-AFP rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 68 kDaObserved band size: 68 kDa

Applications for alpha-Fetoprotein/AFP Antibody (003)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Fetoprotein/AFP

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Alternate Names

AFP, DSCAM2

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents for alpha-Fetoprotein/AFP Antibody (003)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for alpha-Fetoprotein/AFP Antibody (003)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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