alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70103

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2A9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all alpha-Fetoprotein/AFP Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

66.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9)Azide and BSA Free [NBP2-70103]

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9)Azide and BSA Free [NBP2-70103]

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha 1 Fetoprotein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Al
Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103]

Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103]

Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Alpha 1 Fetoprotein.
Immunohistochemistry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103]

Immunohistochemistry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103]

Immunohistochemistry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Staining of paraffin-embedded Human liver tissue using anti-Alpha 1 Fetoprotein mouse monoclonal antibody.
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Applications for alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Fetoprotein/AFP

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Alternate Names

AFP, DSCAM2

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents for alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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