alpha Glucosidase 2 Antibody (PSH01-52)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32366
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-52
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human alpha Glucosidase 2 aa 29-450. (Uniprot: Q14697)
Localization
Endoplasmic reticulum, Golgi apparatus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for alpha Glucosidase 2 Antibody (PSH01-52)
Immunoprecipitation: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366]
Immunoprecipitation: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366] - alpha Glucosidase 2 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32366 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32366 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.Lane 1: HeLa cell lysate (input)
Lane 2: Rabbit IgG instead of NBP3-32366 in HeLa cell lysate
Lane 3: NBP3-32366 IP in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 21 seconds
Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366]
Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32366) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32366) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366]
Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366] - Immunofluorescence analysis of paraffin-embedded human thyroid tissue labeling alpha Glucosidase 2 with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32366) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32366, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Applications for alpha Glucosidase 2 Antibody (PSH01-52)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000
Immunoprecipitation
1-2ug/sample
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha Glucosidase 2
Alternate Names
Alpha-glucosidase 2, EC 3.2.1, EC 3.2.1.84, G2ANGLUII, Glucosidase II subunit alpha, glucosidase, alpha; neutral AB, GluII, KIAA0088alpha glucosidase II alpha subunit, neutral alpha-glucosidase AB
Gene Symbol
GANAB
Additional alpha Glucosidase 2 Products
Product Documents for alpha Glucosidase 2 Antibody (PSH01-52)
Product Specific Notices for alpha Glucosidase 2 Antibody (PSH01-52)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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