alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16091

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16091-100ul
NBP3-16091-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7X5Z2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all alpha-Methylacyl-CoA Racemase/AMACR Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human alpha-Methylacyl-CoA Racemase/AMACR (NP_055139.4). MALQGISVVELSGLAPGPFCAMVLADFGARVVRVDRPGSRYDVSRLGRGKRSLVLDLKQPRGAAVLRRLCKRSDVLLEPFRRGVMEKLQLGPEILQRENPRLIYARLSGFGQSGSFCRLAGHDINYLALSGVLSKIGR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2)

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2) [NBP3-16091]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] - Immunohistochemistry of paraffin-embedded human prostate cancer using alpha-Methylacyl-CoA Racemase/AMACR Rabbit mAb (NBP3-16091) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10)

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] -

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] - Analysis of various lysates, using AMACR antibody at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10)

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] -

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (4R3L10) [NBP3-16091] - Analysis of paraffin-embedded human kidney using AMACR Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Alternate Names

alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE

Gene Symbol

AMACR

Additional alpha-Methylacyl-CoA Racemase/AMACR Products

Product Documents for alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-Methylacyl-CoA Racemase/AMACR Antibody (7X5Z2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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