alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54501

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # AMACR/1723

Format

Azide and BSA Free

Concentration

1 mg/ml

View all alpha-Methylacyl-CoA Racemase/AMACR Antibodies »

Product Specifications

Immunogen

Recombinant full-length human alpha-Methylacyl-CoA Racemase/AMACR protein (Uniprot: Q9UHK6)

Localization

Cytoplasmic

Marker

Prostate Cancer Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53390).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723)Azide and BSA Free [NBP2-54501]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723)Azide and BSA Free [NBP2-54501]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot analysis of Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/1723).
Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma Stained with AMACR / p504S Monoclonal Antibody (AMACR/1723)
Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723)Azide and BSA Free [NBP2-54501]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723)Azide and BSA Free [NBP2-54501]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723) - Azide and BSA Free [NBP2-54501] - Western Blot Analysis of PC3 cell lysate using alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/1723).
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Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Alternate Names

alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE

Gene Symbol

AMACR

Additional alpha-Methylacyl-CoA Racemase/AMACR Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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