alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45491

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5F10

Concentration

1 mg/ml

View all alpha-Methylacyl-CoA Racemase/AMACR Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human AMACR (NP_055139) produced in HEK293T cell.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30067967) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10)

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMACR.
Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491]

Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491]

Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) [NBP2-45491] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Applications for alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Alternate Names

alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE

Gene Symbol

AMACR

Additional alpha-Methylacyl-CoA Racemase/AMACR Products

Product Documents for alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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