alpha-Sarcoglycan Antibody (JA51-81)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67150

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67150
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA51-81

Concentration

1 mg/ml

View all alpha-Sarcoglycan Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human alpha-Sarcoglycan aa 338-387 / 387. (SwissProt: Q16586 Human; SwissProt: P82350 Mouse; SwissProt: Q5SWB2 Rat)

Localization

Sarcolemma, cytoskeleton.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha-Sarcoglycan Antibody (JA51-81)

Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150]

Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150]

Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Western blot analysis of alpha-Sarcoglycan on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse heart tissue lysate Lane 2: human skeletal muscle tissue lysate
Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150]

Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150]

Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150]

Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150]

Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.
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Applications for alpha-Sarcoglycan Antibody (JA51-81)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Application Notes
Alpha-Sarcoglycan Antibody (JA51-81) validated for IHC-Fr from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-67150 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Sarcoglycan

The dystrophin-glycoprotein complex (DGC) comprises a group of proteins that are critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Components of the DGC include dystrophin (MIM 300377), which is deficient in Duchenne muscular dystrophy (DMD; MIM 310200); syntrophins (e.g., MIM 600026); dystroglycans (MIM 128239); and sarcoglycans, such as adhalin, a 50-kD transmembrane protein (Roberds et al., 1993 [PubMed 8226900]).[supplied by OMIM]

Alternate Names

50-DAG, Adhalin, ADL, alphaSarcoglycan, DAG2, DMDA2, Dystroglycan-2, LGMD2D, SCARMD1, SGCA

Gene Symbol

SGCA

Additional alpha-Sarcoglycan Products

Product Documents for alpha-Sarcoglycan Antibody (JA51-81)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-Sarcoglycan Antibody (JA51-81)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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