alpha-Sarcoglycan Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90306

Novus Biologicals, part of Bio-Techne
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NBP1-90306
NBP1-90306-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alpha-Sarcoglycan Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SALDRGGRVPLPIEGRKEGVYIKVGSASPFSTCLKMVASPDSHARCAQGQPPLLSCYDTLAPHFRVDWCNVTLVDKSVPEPADEVPTPGDGILEHDPFFCPPTEAPDRDFLVD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha-Sarcoglycan Antibody

Western Blot: alpha-Sarcoglycan Antibody [NBP1-90306]

Western Blot: alpha-Sarcoglycan Antibody [NBP1-90306]

Western Blot: alpha-Sarcoglycan Antibody [NBP1-90306] - Analysis in control (vector only transfected HEK293T lysate) and SGCA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody [NBP1-90306]

Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody [NBP1-90306]

Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody [NBP1-90306] - Staining of human heart muscle shows distinct positivity of intercalated discs in myocytes.

Applications for alpha-Sarcoglycan Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Sarcoglycan

The dystrophin-glycoprotein complex (DGC) comprises a group of proteins that are critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Components of the DGC include dystrophin (MIM 300377), which is deficient in Duchenne muscular dystrophy (DMD; MIM 310200); syntrophins (e.g., MIM 600026); dystroglycans (MIM 128239); and sarcoglycans, such as adhalin, a 50-kD transmembrane protein (Roberds et al., 1993 [PubMed 8226900]).[supplied by OMIM]

Alternate Names

50-DAG, Adhalin, ADL, alphaSarcoglycan, DAG2, DMDA2, Dystroglycan-2, LGMD2D, SCARMD1, SGCA

Entrez Gene IDs

6442 (Human)

Gene Symbol

SGCA

UniProt

Q16586

Additional alpha-Sarcoglycan Products

Product Documents for alpha-Sarcoglycan Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-Sarcoglycan Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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