alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34760

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Chicken, Feline, Goat, Guinea Pig, Monkey, Rabbit, Sheep

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SPM332

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all alpha-Smooth Muscle Actin Antibodies »

Product Specifications

Immunogen

N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH. (Uniprot: P62736)

Localization

Cytoplasmic

Specificity

Actin is a major component of the cytoskeleton and is present in most cell types. This monoclonal antibody is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This monoclonal antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Marker

Leiomyosarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32808).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free [NBP2-34760]

Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free [NBP2-34760]

Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free [NBP2-34760] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).
Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free [NBP2-34760]

Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free [NBP2-34760]

Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free [NBP2-34760] - Livers were harvested at 9 wpi, formalin-fixed, and then cryosectioned and stained for markers of inflammation and liver fibrosis. Maximum intensity projections of 12-17 um thick liver sections with immunofluorescently labeled nuclei (DAPI white) alpha-smooth muscle actin NBP2-34760APC (yellow), CD45 (red) and Collagen I NB600-408 (top) or Collagen III/COL3A1 NB600-594 (bottom) (blue) in the liver of uninfected or 9 wpi WT mice. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32973293/) licensed under a CC-BY license.
Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free [NBP2-34760]

Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free [NBP2-34760]

Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free [NBP2-34760] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin. Image from the Allophycocyanin version of this antibody.
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Applications for alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-Smooth Muscle Actin Antibody (SPM332) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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