alpha-Synuclein Antibody (AS11) [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # DDX0910-HRPO-100
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
HRP
Antibody Source
Monoclonal Mouse IgG Clone # AS11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This alpha-Synuclein Antibody (AS11) was developed against Human alpha-synuclein.
Specificity
This alpha-Synuclein Antibody (AS11) is specific for the N-terminal portion of human a-synuclein (aa 1-60).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for alpha-Synuclein Antibody (AS11) [HRP]
Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (AS11) [HRP] [DDX0910-HRPO-100] - IF staining of human neuroblastoma (SH-SY5Y) expressing endogenousa-synuclein
Immunohistochemistry: alpha-Synuclein Antibody (AS11) [HRP] [DDX0910-HRPO-100] - IHC staining of pathological human brains with AS11
Applications for alpha-Synuclein Antibody (AS11) [HRP]
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Formulation, Preparation, and Storage
Purification
Ion exchange chromatography
Formulation
PBS and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Background: alpha-Synuclein
A number of studies have revealed that alpha-synuclein aggregation is a hallmark feature in a number of neurodegenerative diseases, referred to as synucleinopathies (2-4). Alpha-synuclein protein aggregates are a large component of Lewy bodies that are present in Parkinson's disease (PD), Lewy body dementia (LBD), and multiple system atrophy (1-6). Research has shown phosphorylation of alpha-synuclein at Ser129 moves the protein from the nucleus to the cytoplasm and promotes fibril formation associated with synucleinopathies (1,2,5). Recent studies also suggest that alpha-synuclein accumulation can prevent mitochondrial import machinery causing mitochondrial dysfunction that is often observed in neurodegeneration (5). It is thought that preventing alpha-synuclein aggregation may prevent PD, thus alpha-synuclein is a target for many potential therapeutic interventions aimed at decreasing aggregate formation or increasing clearance (1,2,4-6).
References
1. Villar-Pique, A., Lopes da Fonseca, T., & Outeiro, T. F. (2016). Structure, function and toxicity of alpha-synuclein: the Bermuda triangle in synucleinopathies. Journal of neurochemistry. https://doi.org/10.1111/jnc.13249
2. Emamzadeh F. N. (2016). Alpha-synuclein structure, functions, and interactions. Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences. https://doi.org/10.4103/1735-1995.181989
3. Burre J. (2015). The Synaptic Function of alpha-Synuclein. Journal of Parkinson's disease. https://doi.org/10.3233/JPD-150642
4. Lashuel, H. A., Overk, C. R., Oueslati, A., & Masliah, E. (2013). The many faces of alpha-synuclein: from structure and toxicity to therapeutic target. Nature reviews. Neuroscience. https://doi.org/10.1038/nrn3406
5. Rocha, E. M., De Miranda, B., & Sanders, L. H. (2018). Alpha-synuclein: Pathology, mitochondrial dysfunction and neuroinflammation in Parkinson's disease. Neurobiology of disease. https://doi.org/10.1016/j.nbd.2017.04.004
6. O'Leary, E. I., & Lee, J. C. (2019). Interplay between alpha-synuclein amyloid formation and membrane structure. Biochimica et biophysica acta. Proteins and proteomics. https://doi.org/10.1016/j.bbapap.2018.09.012
Alternate Names
NACP, PARK1, PARK4, SNCA, Synuclein-alpha
Gene Symbol
SNCA
Additional alpha-Synuclein Products
Product Documents for alpha-Synuclein Antibody (AS11) [HRP]
Product Specific Notices for alpha-Synuclein Antibody (AS11) [HRP]
This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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