Key Product Details

Species Reactivity

Human, Mouse

Applications

Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # J18

Concentration

1 mg/ml

View all alpha-Synuclein Antibodies »

Product Specifications

Immunogen

Human alpha-Synuclein AA 124-134: AYEMP-pS-EEGYQ-Cys

Modification

p Ser129, Cys Glu134

Localization

Cytoplasm|Cytosol|Membrane|Nucleus|Cell Junction|Synapse

Specificity

Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha-Synuclein [Cys Glu134, p Ser129] Antibody (J18)

Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.
Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (C) 5 ug alpha synuclein PFFs Inset: PBS (negative control). Primary antibody: Anti-alpha-Synuclein (NBP3-18266) at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site.
Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Mouse Brain showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Multiple bands due to phosphorylation.
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Applications for alpha-Synuclein [Cys Glu134, p Ser129] Antibody (J18)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Optimal dilutions for assays should be determined by the user.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS pH 7.4, 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Synuclein

Alpha-synuclein, a member of the synuclein family, is a protein that was first identified in 1988 whose name is derived from its localization to both the synapse and nucleus (1-3). Specifically, it is expressed primarily in the brain, including Lewy Bodies (1-6). Alpha-synuclein is encoded by the SNCA gene, located on chromosome 4p21, and is processed as a 140 amino acid (aa) protein with a theoretical molecular weight of 14 kDa (1,2,4). Structurally alpha-synuclein consists of a N-terminal binding domain (1-60 aa), a central domain core region called the non-amyloid-beta component (NAC) (61-95 aa), and a C-terminal domain (96-140 aa) (1-3). The N-terminal region contains aa repeats with a KTKEGV consensus sequence that gives the protein its alpha-helical structure that associates with lipid membranes (1-4). The hydrophobic NAC region is responsible for alpha-synuclein aggregation and fibril formation (1-4). The acidic C-terminal tail is largely unstructured but can be targeted for post-translational modifications (1-4). The function of alpha-synuclein is not entirely understood, but it is shown to have a role in suppression of apoptosis, acting as a molecular chaperone, regulating glucose, and modulating calmodulin activity (1,3).

A number of studies have revealed that alpha-synuclein aggregation is a hallmark feature in a number of neurodegenerative diseases, referred to as synucleinopathies (2-4). Alpha-synuclein protein aggregates are a large component of Lewy bodies that are present in Parkinson's disease (PD), Lewy body dementia (LBD), and multiple system atrophy (1-6). Research has shown phosphorylation of alpha-synuclein at Ser129 moves the protein from the nucleus to the cytoplasm and promotes fibril formation associated with synucleinopathies (1,2,5). Recent studies also suggest that alpha-synuclein accumulation can prevent mitochondrial import machinery causing mitochondrial dysfunction that is often observed in neurodegeneration (5). It is thought that preventing alpha-synuclein aggregation may prevent PD, thus alpha-synuclein is a target for many potential therapeutic interventions aimed at decreasing aggregate formation or increasing clearance (1,2,4-6).

References

1. Villar-Pique, A., Lopes da Fonseca, T., & Outeiro, T. F. (2016). Structure, function and toxicity of alpha-synuclein: the Bermuda triangle in synucleinopathies. Journal of neurochemistry. https://doi.org/10.1111/jnc.13249

2. Emamzadeh F. N. (2016). Alpha-synuclein structure, functions, and interactions. Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences. https://doi.org/10.4103/1735-1995.181989

3. Burre J. (2015). The Synaptic Function of alpha-Synuclein. Journal of Parkinson's disease. https://doi.org/10.3233/JPD-150642

4. Lashuel, H. A., Overk, C. R., Oueslati, A., & Masliah, E. (2013). The many faces of alpha-synuclein: from structure and toxicity to therapeutic target. Nature reviews. Neuroscience. https://doi.org/10.1038/nrn3406

5. Rocha, E. M., De Miranda, B., & Sanders, L. H. (2018). Alpha-synuclein: Pathology, mitochondrial dysfunction and neuroinflammation in Parkinson's disease. Neurobiology of disease. https://doi.org/10.1016/j.nbd.2017.04.004

6. O'Leary, E. I., & Lee, J. C. (2019). Interplay between alpha-synuclein amyloid formation and membrane structure. Biochimica et biophysica acta. Proteins and proteomics. https://doi.org/10.1016/j.bbapap.2018.09.012

Alternate Names

NACP, PARK1, PARK4, SNCA, Synuclein-alpha

Gene Symbol

SNCA

Additional alpha-Synuclein Products

Product Documents for alpha-Synuclein [Cys Glu134, p Ser129] Antibody (J18)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-Synuclein [Cys Glu134, p Ser129] Antibody (J18)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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