alpha Tubulin Antibody (DM1AR)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38646
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Fungi, Goat, Guinea Pig, Hamster, Monkey, Parasite, Primate, Rabbit, Xenopus, Yeast
Applications
Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # DM1AR
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This Recombinant alpha Tubulin Antibody (DM1AR) was developed against native chicken brain microtubules.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:34871568) Use in Mouse reported in scientific literature (PMID:34533563). Yeast reactivity reported in scientific literature (PMID: 25126732). Goat reactivity reported in scientific literature (PMID:31805146). Will likely react with all mammals.
Marker
Microtubule Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for alpha Tubulin Antibody (DM1AR)
Detection of alpha Tubulin (DM1AR) in U-251 MG Human Cell Line by Flow Cytometry.
An intracellular stain was performed on immersion fixed U-251 MG human glioblastoma cell line with Mouse anti-alpha Tubulin (DM1AR) Protein-G purified Recombinant Monoclonal Antibody (Catalog # NBP3-38646, blue histogram) or matched control antibody (Catalog # MAB002, orange histogram). Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature.
alpha Tubulin (DM1AR) in U-2 OS Human Cell Line.
alpha Tubulin (DM1AR) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-alpha Tubulin (DM1AR) Protein G-purified Recombinant Monoclonal Antibody (Catalog # NBP3-38646) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Mouse IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
alpha Tubulin (DM1AR) in NIH-3T3 Mouse Cell Line.
alpha Tubulin (DM1AR) was detected in immersion fixed NIH3T3 mouse fibroblast cell line using Mouse anti-alpha Tubulin (DM1AR) Protein G-purified Recombinant Monoclonal Antibody (Catalog # NBP3-38646) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Mouse IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.Applications for alpha Tubulin Antibody (DM1AR)
Application
Recommended Usage
Flow (Intracellular)
1 ug per million cells
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
1 ug/ml
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1:5000
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha Tubulin
Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).
/>References
/>1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921
2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718
Long Name
Tubulin Alpha 1a
Alternate Names
Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1, alpha tubulin loading control, alpha tubulin monoclonal, DM1A, DM1A anti-tubulin, dm1a monoclonal, DM1a tubulin, TUBA1 monoclonal, tubulin monoclonal
Gene Symbol
TUBA1A
Additional alpha Tubulin Products
Product Documents for alpha Tubulin Antibody (DM1AR)
Product Specific Notices for alpha Tubulin Antibody (DM1AR)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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