AlphaA Crystallin/CRYAA Antibody (1H3.B8)

Western Blot: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875]

Western Blot: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Western Blot analysis of Bovine tissue lysate showing detection of AlphaA Crystallin/CRYAA protein using Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody, Clone 1H3.B8 (NBP2-12875). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody (NBP2-12875) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. This blot shows absolute specificity as Left: AlphaA Crystallin/CRYAA, Right: Alpha B Crystallin.

Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875]

Immunocytochemistry/Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody, Clone 1H3.B8 (NBP2-12875). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody (NBP2-12875) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) AlphaA Crystallin/CRYAA Antibody. (D) Composite.

Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875]

Immunocytochemistry/Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha A Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X.