AlphaA Crystallin/CRYAA Antibody (c9F2)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-14821

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # c9F2

Concentration

1.0 mg/ml

View all AlphaA Crystallin/CRYAA Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant protein corresponding to full-length (aa1-173) human crystallin alpha A

Reactivity Notes

Predicted cross-reactivities: Rat

Specificity

NB120-14821 recognizes alpha A crystallin, a 20kDa water-soluble major structural protein and member of the heat shock protein family, designated HSPB4, which acts as a molecular chaperone and is believed to play a role in the maintenance of ocular lens transparency/refractive index. Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for AlphaA Crystallin/CRYAA Antibody (c9F2)

Western Blot: AlphaA Crystallin/CRYAA Antibody (c9F2)BSA Free [NB120-14821]

Western Blot: AlphaA Crystallin/CRYAA Antibody (c9F2)BSA Free [NB120-14821]

Western Blot: AlphaA Crystallin/CRYAA Antibody (c9F2) [NB120-14821] - Analysis of recombinant crystallin proteins and mouse eye derived lysates probed with Mouse anti Human alpha crystallin.

Applications for AlphaA Crystallin/CRYAA Antibody (c9F2)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000-1:2000
Application Notes
NB120-14821 detects alpha A Crystallin from rat eye extracts at a dilution of 1:1000. Proteins are visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AlphaA Crystallin/CRYAA

Alpha crystallins are major proteins of the ocular lens and are thought to play a role in maintaining lens transparency. They are composed of two gene products alpha A and alpha B, for acidic and basic, respectively. Alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP) family. They act as molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of 30-40 subunits of alpha crystallins, and the alpha A and alpha B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and the participation in the intracellular architecture.

Alternate Names

CRYA1, CRYAA, HSPB4

Entrez Gene IDs

1409 (Human); 24273 (Rat)

Gene Symbol

CRYAA

UniProt

P02489 (Human)

Additional AlphaA Crystallin/CRYAA Products

Product Documents for AlphaA Crystallin/CRYAA Antibody (c9F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AlphaA Crystallin/CRYAA Antibody (c9F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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