AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70450

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3B12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all AlphaA Crystallin/CRYAA Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CRYAA(NP_000385) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free

Western Blot: AlphaA Crystallin/CRYAA Antibody (OTI3B12)Azide and BSA Free [NBP2-70450]

Western Blot: AlphaA Crystallin/CRYAA Antibody (OTI3B12)Azide and BSA Free [NBP2-70450]

Western Blot: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAA.
Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450]

Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450]

Immunocytochemistry/Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CRYAA.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450]

Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450]

Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free [NBP2-70450] - Analysis of Human prostate tissue.
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Applications for AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AlphaA Crystallin/CRYAA

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander®s disease where it accumulates in brain cells of those afflicted.

Alternate Names

CRYA1, CRYAA, HSPB4

Gene Symbol

CRYAA

Additional AlphaA Crystallin/CRYAA Products

Product Documents for AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AlphaA Crystallin/CRYAA Antibody (OTI3B12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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