AlphaA Crystallin/CRYAA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-5595

Novus Biologicals, part of Bio-Techne
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NB120-5595
NB120-5595-0.025mg
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Key Product Details

Species Reactivity

Rat, Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all AlphaA Crystallin/CRYAA Antibodies »

Product Specifications

Immunogen

Purified alpha-A Crystallin from bovine lens.

Localization

Cytoplasmic

Specificity

This does not detect the unphosphorylated form of the protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AlphaA Crystallin/CRYAA Antibody

Western Blot: AlphaA Crystallin/CRYAA Antibody [NB120-5595]

Western Blot: AlphaA Crystallin/CRYAA Antibody [NB120-5595]

Western Blot: AlphaA Crystallin/CRYAA Antibody [NB120-5595] - Analysis of alpha-A crystallin from bovine lens extract.

Applications for AlphaA Crystallin/CRYAA Antibody

Application
Recommended Usage

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

0.1 ug/ml
Application Notes
WB: Detects an approx. 20 kDa representing alpha-A crystallin from bovine lens samples. IHC: Staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute to desired concentration.

Formulation

PBS with 30 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AlphaA Crystallin/CRYAA

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.

Alternate Names

CRYA1, CRYAA, HSPB4

Entrez Gene IDs

1409 (Human); 24273 (Rat)

Gene Symbol

CRYAA

Additional AlphaA Crystallin/CRYAA Products

Product Documents for AlphaA Crystallin/CRYAA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AlphaA Crystallin/CRYAA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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