ALPPL2 Antibody (2B3)
Novus Biologicals, part of Bio-Techne | Catalog # H00000251-M07
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Sandwich ELISA, Sandwich ELISA Capture, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2B3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ALPPL2 (NP_112603, 365 a.a. ~ 454 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGE
Specificity
ALPPL2 - alkaline phosphatase, placental-like 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for ALPPL2 Antibody (2B3)
Western Blot: ALPPL2 Antibody (2B3) [H00000251-M07]
Western Blot: ALPPL2 Antibody (2B3) [H00000251-M07] - Analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody (M07), clone 2B3. Lane 1: ALPPL2 transfected lysatE (57.5 KDa). Lane 2: Non-transfected lysate.Immunohistochemistry-Paraffin: ALPPL2 Antibody (2B3) [H00000251-M07]
Immunohistochemistry-Paraffin: ALPPL2 Antibody (2B3) [H00000251-M07] - Analysis of monoclonal antibody to ALPPL2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/mlWestern Blot: ALPPL2 Antibody (2B3) [H00000251-M07]
Western Blot: ALPPL2 Antibody (2B3) [H00000251-M07] - ALPPL2 over-expressed 293 cell line. Lane 2 cotransfected with ALPPL2 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000251-M07. GAPDH (36.1 kDa) used as loading control.Applications for ALPPL2 Antibody (2B3)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ALPPL2
Alternate Names
Alkaline phosphatase Nagao isozyme, alkaline phosphatase, placental-like, alkaline phosphatase, placental-like 2, ALP-1, ALPG, ALPPL, EC 3.1.3.1, GCAP, Germ cell alkaline phosphatase, Nagao isozyme, Placental alkaline phosphatase-like, placental-like alkaline phosphatase, PLAP-like, testicular and thymus alkaline phosphatase
Entrez Gene IDs
251 (Human)
Gene Symbol
ALPG
OMIM
171810 (Human)
UniProt
Additional ALPPL2 Products
Product Documents for ALPPL2 Antibody (2B3)
Product Specific Notices for ALPPL2 Antibody (2B3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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