Aly Antibody (JE36-45)

Western Blot: Aly Antibody (JE36-45) [NBP3-32033]

Western Blot: Aly Antibody (JE36-45) [NBP3-32033] - Western blot analysis of Aly on different lysates with Rabbit anti-Aly antibody (NBP3-32033) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: K-562 cell lysate (20 ug/Lane)
Lane 3: 293T cell lysate (20 ug/Lane)
Lane 4: A549 cell lysate (20 ug/Lane)
Lane 5: SK-OV-3 cell lysate (20 ug/Lane)
Lane 6: HL-60 cell lysate (20 ug/Lane)
Lane 7: NIH/3T3 cell lysate (20 ug/Lane)
Lane 8: Neuro-2a cell lysate (20 ug/Lane)
Lane 9: C6 cell lysate (20 ug/Lane)
Lane 10: Mouse testis tissue lysate (40 ug/Lane)
Lane 11: Mouse spleen tissue lysate (40 ug/Lane)
Lane 12: Rat testis tissue lysate (40 ug/Lane)

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32033) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Aly Antibody (JE36-45) [NBP3-32033]

Immunohistochemistry: Aly Antibody (JE36-45) [NBP3-32033] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Aly antibody (NBP3-32033) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32033) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Aly Antibody (JE36-45) [NBP3-32033]

Immunocytochemistry/ Immunofluorescence: Aly Antibody (JE36-45) [NBP3-32033] - Immunocytochemistry analysis of HepG2 cells labeling Aly with Rabbit anti-Aly antibody (NBP3-32033) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aly antibody (NBP3-32033) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.