AMBRA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24723

Novus Biologicals, part of Bio-Techne
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NBP2-24723
NBP2-24723SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Chicken

Predicted:

Canine (100%), Chimpanzee (100%), Monkey (100%), Opossum (100%), Porcine (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all AMBRA1 Antibodies »

Product Summary for AMBRA1 Antibody

Immunogen

A portion of amino acids 1-50 of human Ambra1 was used as the immunogen for the antibody.

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is 100% homologous in Macaques.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMBRA1 Antibody

Western Blot: AMBRA1 Antibody [NBP2-24723]

Western Blot: AMBRA1 Antibody [NBP2-24723]

Western Blot: AMBRA1 Antibody [NBP2-24723] - analysis of Ambra1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using Ambra1 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-24723]

Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-24723]

Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-24723] - Formalin-fixed, paraffin-embedded human small intestine stained with Ambra1 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Applications for AMBRA1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMBRA1

AMBRA1 regulates autophagy and development of the nervous system. It is a large, previously unknown protein bearing a WD40 domain at its amino terminus, regulates autophagy and has a crucial role in embryogenesis. AMBRA1 is a positive regulator of the Becn1-dependent programme of autophagy, as revealed by its overexpression and by RNA interference experiments invitro. Notably, AMBRA1 functional deficiency in mouse embryos leads to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic cell death.

Alternate Names

activating molecule in BECN1-regulated autophagy protein 1, autophagy/beclin-1 regulator 1, DCAF3, DDB1 and CUL4 associated factor 3, FLJ20294, KIAA1736activating molecule in beclin-1-regulated autophagy, MGC33725, WD repeat domain 94, WDR94

Gene Symbol

AMBRA1

UniProt

Q9C0C7 (Human)

Additional AMBRA1 Products

Product Documents for AMBRA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMBRA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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