Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S86-36

Concentration

1 mg/ml

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Product Specifications

Immunogen

Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1

Localization

Cell Membrane , Cell Projection , Axon

Specificity

Detects approx 60-80kDa depending on maturity/glycosylation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for AMIGO Antibody (S86-36)

Western Blot: AMIGO Antibody (S86-36) [NBP2-22413]

Western Blot: AMIGO Antibody (S86-36) [NBP2-22413]

Western Blot: AMIGO Antibody (S86-36) [NBP2-22413] - Western Blot analysis of Rat Brain Membrane showing detection of 60-80 kDa AMIGO-1 protein using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (NBP2-22413). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (NBP2-22413) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 60-80 kDa.
Immunocytochemistry/ Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413]

Immunocytochemistry/ Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413]

Immunocytochemistry/Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (NBP2-22413). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (NBP2-22413) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) AMIGO-1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413]

Immunocytochemistry/ Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413]

Immunocytochemistry/Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.
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Applications for AMIGO Antibody (S86-36)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
Use in Flow reported in scientific literature (PMID:35273332) 1 ug/ml of AMIGO-1 Antibody was sufficient for detection of AMIGO-1 in 20 ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody..

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMIGO

Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved infasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neuralplasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction andcontribute to signal transduction through its intracellular domain

Long Name

Amphoterin-induced Gene and ORF

Alternate Names

ali2, Alivin-2, AMIGO1

Gene Symbol

AMIGO1

UniProt

Q86WK6

Additional AMIGO Products

Product Documents for AMIGO Antibody (S86-36)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMIGO Antibody (S86-36)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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