Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6F4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Aminomethyltransferase Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 146-403 of human AMT(NP_000472) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free

Western Blot: Aminomethyltransferase Antibody (OTI6F4)Azide and BSA Free [NBP2-70171]

Western Blot: Aminomethyltransferase Antibody (OTI6F4)Azide and BSA Free [NBP2-70171]

Western Blot: Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free [NBP2-70171] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aminomethyltransferase.
Immunohistochemistry: Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free [NBP2-70171]

Immunohistochemistry: Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free [NBP2-70171]

Immunohistochemistry: Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free [NBP2-70171] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free [NBP2-70171]

Immunohistochemistry: Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free [NBP2-70171]

Immunohistochemistry: Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free [NBP2-70171] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aminomethyltransferase

The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.[supplied by OMIM]

Alternate Names

aminomethyltransferase, EC 2.1.2.10, GCE, GCSTaminomethyltransferase (glycine cleavage system protein T), glycine cleavage system protein T, NKHmitochondrial

Gene Symbol

AMT

Additional Aminomethyltransferase Products

Product Documents for Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aminomethyltransferase Antibody (OTI6F4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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