Aminomethyltransferase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89783

Novus Biologicals, part of Bio-Techne
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NBP1-89783
NBP1-89783-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Aminomethyltransferase Antibodies »

Product Summary for Aminomethyltransferase Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminomethyltransferase Antibody

Western Blot: Aminomethyltransferase Antibody [NBP1-89783]

Western Blot: Aminomethyltransferase Antibody [NBP1-89783]

Western Blot: Aminomethyltransferase Antibody [NBP1-89783] - Analysis in human cell line HepG2.
Immunocytochemistry/ Immunofluorescence: Aminomethyltransferase Antibody [NBP1-89783]

Immunocytochemistry/ Immunofluorescence: Aminomethyltransferase Antibody [NBP1-89783]

Immunocytochemistry/Immunofluorescence: Aminomethyltransferase Antibody [NBP1-89783] - Staining of human cell line HeLa shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Aminomethyltransferase Antibody [NBP1-89783]

Immunohistochemistry-Paraffin: Aminomethyltransferase Antibody [NBP1-89783]

Immunohistochemistry-Paraffin: Aminomethyltransferase Antibody [NBP1-89783] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Applications for Aminomethyltransferase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aminomethyltransferase

The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.[supplied by OMIM]

Alternate Names

aminomethyltransferase, EC 2.1.2.10, GCE, GCSTaminomethyltransferase (glycine cleavage system protein T), glycine cleavage system protein T, NKHmitochondrial

Entrez Gene IDs

275 (Human)

Gene Symbol

AMT

UniProt

P48728 (Human)

Additional Aminomethyltransferase Products

Product Documents for Aminomethyltransferase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Aminomethyltransferase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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