Aminopeptidase A/ENPEP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1885

Novus Biologicals, part of Bio-Techne
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NB100-1885
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Aminopeptidase A/ENPEP Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EQYQKTSLAQEKEK corresponding to internal region according to NP_001968.3.

Reactivity Notes

Rat reactivity in WB reported in scientific literature (PMID: 28399178).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Aminopeptidase A/ENPEP Antibody

Western Blot: Aminopeptidase A/ENPEP Antibody [NB100-1885]

Western Blot: Aminopeptidase A/ENPEP Antibody [NB100-1885]

Western Blot: Aminopeptidase A/ENPEP Antibody [NB100-1885] - Staining of human testis lysate (RIPA buffer, 30 ug total protein). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Aminopeptidase A/ENPEP Antibody [NB100-1885]

Immunohistochemistry-Paraffin: Aminopeptidase A/ENPEP Antibody [NB100-1885]

Immunohistochemistry-Paraffin: Aminopeptidase A/ENPEP Antibody [NB100-1885] - Staining of paraffin embedded Human Kidney. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Aminopeptidase A/ENPEP Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/mL

Immunohistochemistry-Paraffin

3 - 5 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx 170 kDa band observed in human brain and testis lysates (calculated MW of 109 kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 11994381). IHC: Human kidney shows staining of basement membrane and the mesangium in the glomerulus.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aminopeptidase A/ENPEP

Aminopeptidase A is a zinc-dependent metallopeptidase that selectively cleaves N-terminal Glu, or Asp residues from peptides. In mice, it is expressed on early B cells and on a subset of thymic cortical epithelial cells, but not on mature lymphocytes. Additionally, it is expressed on endothelial cells, intestinal epithelium, proximal renal tubules, and other non-hemopoietic tissues. In humans, angiotensin II is the the best-defined biologically active substrate of APA. Although its role in the immune response is still uncertain, its expression on renal cell carcinoma has been the focus of some cancer research studies.

Alternate Names

CD249, ENPEP

Entrez Gene IDs

2028 (Human); 13809 (Mouse)

Gene Symbol

ENPEP

UniProt

Q07075 (Human)

Additional Aminopeptidase A/ENPEP Products

Product Documents for Aminopeptidase A/ENPEP Antibody

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Product Specific Notices for Aminopeptidase A/ENPEP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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