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Aminopeptidase A/ENPEP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32442

Novus Biologicals, part of Bio-Techne
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NBP1-32442

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Porcine (90%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Aminopeptidase A/ENPEP. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%), Bovine (89%).

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

109 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminopeptidase A/ENPEP Antibody

Western Blot: Aminopeptidase A/ENPEP Antibody [NBP1-32442]

Western Blot: Aminopeptidase A/ENPEP Antibody [NBP1-32442]

Western Blot: Aminopeptidase A/ENPEP Antibody [NBP1-32442] - Sample(30 ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Aminopeptidase A/ENPEP Antibody [NBP1-32442]

Immunohistochemistry-Paraffin: Aminopeptidase A/ENPEP Antibody [NBP1-32442]

Immunohistochemistry-Paraffin: Aminopeptidase A/ENPEP Antibody [NBP1-32442] - Paraffin-embedded Kidney, using antibody 10 ug/ml.

Applications for Aminopeptidase A/ENPEP Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Aminopeptidase A/ENPEP

Aminopeptidase A is a zinc-dependent metallopeptidase that selectively cleaves N-terminal Glu, or Asp residues from peptides. In mice, it is expressed on early B cells and on a subset of thymic cortical epithelial cells, but not on mature lymphocytes. Additionally, it is expressed on endothelial cells, intestinal epithelium, proximal renal tubules, and other non-hemopoietic tissues. In humans, angiotensin II is the the best-defined biologically active substrate of APA. Although its role in the immune response is still uncertain, its expression on renal cell carcinoma has been the focus of some cancer research studies.

Alternate Names

CD249, ENPEP

Entrez Gene IDs

2028 (Human)

Gene Symbol

ENPEP

UniProt

Additional Aminopeptidase A/ENPEP Products

Product Documents for Aminopeptidase A/ENPEP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aminopeptidase A/ENPEP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.