Amphiphysin/AMPH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-40788

Novus Biologicals, part of Bio-Techne
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NB100-40788
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Amphiphysin/AMPH Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KRKDESRISKAE corresponding to internal region according to NP_001626.1, NP_647477.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Amphiphysin/AMPH Antibody

Western Blot: Amphiphysin/AMPH Antibody [NB100-40788]

Western Blot: Amphiphysin/AMPH Antibody [NB100-40788]

Western Blot: Amphiphysin/AMPH Antibody [NB100-40788] - Western blot analysis of Amphiphysin using NB100-40788 at 0.5ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NB100-40788]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NB100-40788]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NB100-40788] - (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Amphiphysin/AMPH Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.5 ug/ml
Application Notes
WB: Approx. 125 kDa band observed in mouse brain lysates is reported in scientific literature (PMID: 9348539). A minor band of unknown identity was also consistently observed at 45 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human cerebellum shows nuclear and fibrous staning.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Amphiphysin/AMPH

Amphiphysin is associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants (Amphiphysin 1 and Amphiphysin 2) encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.

Alternate Names

AMPH, AMPH1

Entrez Gene IDs

273 (Human); 218038 (Mouse)

Gene Symbol

AMPH

UniProt

NP_001626.1 (Human)

Additional Amphiphysin/AMPH Products

Product Documents for Amphiphysin/AMPH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Amphiphysin/AMPH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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