Amphiphysin/AMPH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86033

Novus Biologicals, part of Bio-Techne
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NBP1-86033
NBP1-86033-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Amphiphysin/AMPH Antibodies »

Product Summary for Amphiphysin/AMPH Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Amphiphysin/AMPH Antibody

Western Blot: Amphiphysin/AMPH Antibody [NBP1-86033]

Western Blot: Amphiphysin/AMPH Antibody [NBP1-86033]

Western Blot: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human cerebral cortex, colon, kidney and tonsil using Anti-AMPH antibody NBP1-86033 (A) shows similar protein distribution across tissues to independent antibody NBP1-87561 (B).
Immunocytochemistry/ Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-86033]

Immunocytochemistry/ Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-86033]

Immunocytochemistry/Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-86033] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human colon.
View 6 more images

Applications for Amphiphysin/AMPH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Amphiphysin/AMPH

Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Alternate Names

AMPH, AMPH1

Gene Symbol

AMPH

Additional Amphiphysin/AMPH Products

Product Documents for Amphiphysin/AMPH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Amphiphysin/AMPH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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