AMPK alpha 1 Antibody (2B7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22127

Novus Biologicals, part of Bio-Techne
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NBP2-22127
NBP2-22127SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B7

Format

BSA Free

Concentration

1.0 mg/ml

View all AMPK alpha 1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AMPK alpha 1 Antibody (2B7) - BSA Free

Simple Western: AMPK alpha 1 Antibody (2B7)BSA Free [NBP2-22127]

Simple Western: AMPK alpha 1 Antibody (2B7)BSA Free [NBP2-22127]

Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: AMPK alpha 1 Antibody (2B7)BSA Free [NBP2-22127]

Western Blot: AMPK alpha 1 Antibody (2B7)BSA Free [NBP2-22127]

Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 Antibody (2B7) - BSA Free [NBP2-22127]

Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 Antibody (2B7) - BSA Free [NBP2-22127]

Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Applications for AMPK alpha 1 Antibody (2B7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Simple Western

1:50

Western Blot

1:500
Application Notes
This AMPK alpha 1 (2B7) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK alpha 1

AMPK alpha 1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increases the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Entrez Gene IDs

5562 (Human)

Gene Symbol

PRKAA1

UniProt

Q13131

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 Antibody (2B7) - BSA Free

Product Datasheets

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Product Specific Notices for AMPK alpha 1 Antibody (2B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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