AMPK alpha 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49430

Novus Biologicals, part of Bio-Techne
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NBP2-49430
NBP2-49430-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AMPK alpha 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMPK alpha 1 Antibody

Western Blot: AMPK alpha 1 Antibody [NBP2-49430]

Western Blot: AMPK alpha 1 Antibody [NBP2-49430]

Western Blot: AMPK alpha 1 Antibody [NBP2-49430] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Immunohistochemistry: AMPK alpha 1 Antibody [NBP2-49430]

Immunohistochemistry: AMPK alpha 1 Antibody [NBP2-49430]

Immunohistochemistry: AMPK alpha 1 Antibody [NBP2-49430] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Applications for AMPK alpha 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK alpha 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Gene Symbol

PRKAA1

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK alpha 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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